The world of super-resolution imaging is an exciting time for cell biologists, providing an unprecedented clarity that has uncovered the secret lives of cells. Yet with any technique brings its own hurdles to overcome, from data analysis and interpretation, to tackling the physical limits of a sample.
Our goal for this site was to share and exchange super-resolution imaging knowledge with established and potential new users, that hopefully creates new dialogue and partnerships that could explore the potential of further exploiting the applications of these techniques. Here you will also find our custom software and How-to Guide for quantitating super-resolution imaging data, PD-Interpreter, applied in our recently published research in Journal of Biological Chemistry and highlighted as ‘Paper of the Week’
Dr Aylin Hanyaloglu
Senior Lecturer, Imperial College of London
For the past 19 years Dr. Hanyaloglu’s research has focused on understanding fundamental cell biological mechanisms regulating G protein-coupled receptor (GPCR) signal transduction and applying these mechanisms to understand the role of this superfamily of receptors in health and disease. Although her PhD training commenced at the MRC Human Reproductive Sciences Centre, Edinburgh, a move to Australia resulted in her PhD being awarded in 2002, with Distinction, from the University Western Australia (under the supervision of Profs Karin Eidne and Graeme Martin). Her carried out her postdoctoral studies with Prof Mark von Zastrow (UCSF). She joined Imperial College London in 2007 as PI of the GPCR signalling group.
Dr Kim Jonas
Lecturer,St Georges, University of London
Dr Jonas has recently commenced her Lectureship at St Georges, University of London in 2015. She has extensive experience in ovarian function and gonadotrophin hormone receptor action and its impact to reproduction. During her postdoctoral studies (in the groups of Dr. Aylin Hanyaloglu and Prof. Ilpo Huhtaniemi, Imperial College London) she established the super-resolution technology of PD-PALM and development of custom quantitative software PD-Interpreter. Her J Biol Chem publication reporting the first use of dual colour PD-PALM super-resolution imaging of GPCR oligomers was selected by the journal as ‘Paper of the Week’.
PD interpreter is a custom Java-based software package designed to analyse localisation microscopy super resolution data. PD-interpreter utilises the localisation co-ordinates generated by localisation microscopy molecule identifying packages, such as QuickPALM, to analyse cluster patterns that may be present.