Archive for January 2017

Drosophila larva – Sample Preparation for Cryo-SEM

Full credit to the source of this post http://www.leica-microsystems.com/science-lab/drosophila-larva-sample-preparation-for-cryo-sem/

Application Note for Leica EM ACE900 – Drosophila larvae were sandwiched between two 3 mm aluminum slit carriers with the 100 μm cavities facing each other and high-pressure frozen with a Leica EM HPM100. No ethanol as synchronization media was used, 1-hexadecene was used as filler. The wholes of the slit carriers were filled with filter tips dipped in 1-hexadecene to keep the carrier sandwich complete after freezing.

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The Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs

Full credit to the source of this post http://www.leica-microsystems.com/science-lab/hyvolution/the-bif-1-dynamin-2-membrane-fission-machinery-regulates-atg9-containing-vesicle-generation-at-the-rab11-positive-reservoirs/

Application example of HyVolution Super-Resolution – Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are generated through an unknown mechanism and delivered to the autophagosome formation sites. We have previously reported that Atg9-containing membranes undergo continuous tubulation and fission during nutrient starvation in a manner dependent on the curvature-inducing protein Bif-1/Sh3glb1. Here, we identify Dynamin 2 (DNM2) as a Bif-1-interacting protein that mediates the fission of Atg9-containing membranes during autophagy.

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