Gene Editing with CRISPR/Cas9 – Breakthrough in Genome Engineering

http://www.leica-microsystems.com/science-lab/crispr-cas/gene-editing-with-crisprcas9-breakthrough-in-genome-engineering/

The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target sequence via Watson-Crick base pairing. It serves as a marker for the foreign nucleic acid. The second component is the Cas9 protein. It binds to the marked sequence and cuts it due to its nuclease activity. Because the base pairing RNA can be synthesized easily and then used to determine a target region, researchers have utilized this system in the laboratory for genome editing.

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