Structural Study of C. elegans

Full credit to the source of this post http://www.leica-microsystems.com/science-lab/structural-study-of-c-elegans/

Application Note for Leica EM ICE, Leica EM AFS2 – Wildtype L4 stage C. elegans (N2 strain) were placed in the 100 μm deep side of Lecithin-coated (see detailed protocol*) type A 3 mm Cu/Au carriers (Leica) with extracellular filler containing 1% (w/v) Agarose type IX and 2% (w/v) Bovine Serum Albumin in bacteria medium (see preparation details**) and sandwiched with the flat side of Lecithin-coated type B 3 mm Cu/Au carriers (Leica). Samples were frozen in a high-pressure freezer (Leica EM ICE).

Leave a Comment